Meeting Banner
Abstract #2282

Analysis of Vascular Function by DCE-MRI in a Human Endothelial Cell Derived Angiogenesis Model in Mice

Claudia Weidensteiner1, Wilfried Reichardt1, Holger Weber2, Dominik Paul1, Oliver Siedentopf2, Christoph Schaechtele2, Dominik von Elverfeldt1

1Dept. of Diagnostic Radiology, Medical Physics, University Hospital Freiburg, Freiburg, Germany; 2ProQinase GmbH, Freiburg, Germany


Vascular function was studied with dynamic contrast enhanced MRI (DCE-MRI) in a new angiogenesis model in vivo. Human endothelial cells embedded in a Matrigel matrix formed a functional neovasculature after these Matrigel plugs were implanted into mice. DCE-MRI showed that the plugs were perfused which was confirmed by immunohistochemistry. DCE-MRI data were fitted with a two-compartment model. Anti-angiogenic treatment decreased the fitted transfer constants and areas under the enhancement curves as compared to control plugs which indicated a decreased perfusion.