Fernando Jos Bonetto1, Mangala Srinivas1, Arend Heerschap2, Robbie Mailliard3, Eric T. Ahrens4, Carl Figdor5, Jolanda de Vries6
1Tumor Immunology, Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen medical Centre, Nijmegen, Gelderland, Netherlands; 2Radiology, Radboud University Nijmegen Medical Center, Nijmegen, Gelderland, Netherlands; 3Celsense Inc., Pittsburgh, USA; 4Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA; 5Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen Medical Center, Nijmegen, Gelderland, Netherlands; 6Tumor Immunology, Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen Medicla Centre, Nijmegen, Gelderland, Netherlands
Tracking of cells using contrast agents with magnetic resonance imaging provides detailed anatomical information but it lacks quantitative information. A new perfluoropolyether nanoemulsion was studied for labeling of dendritic cells for 19F MRI. Our results demonstrate that the compound is taken up by the cells and does not hamper cell function ( viability, maturation and mRNA uptake).