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Abstract #0906

Detection Limits of Very Small Iron Oxide Nanoparticles in Labeled Cells: A Quantitative Evaluation of Histochemistry and MR-Relaxometry

Gert Klug1, Johannes Bremicker1, Thomas Kampf2, Elisabeth Bauer1, Thomas Basse-Lsebrink2, Meike Weber3, Uwe Gbureck4, Ulrich Nth3, Peter Michael Jakob2, Wolfgang Rudolf Bauer1

1Medizinische Klinik und Poliklinik I, Universittsklinikum Wrzburg, Wrzburg, Germany; 2Experimentelle Physik 5, Universitt Wrzburg, Wrzburg, Germany; 3Orthopdisches Zentrum fr Muskuloskelettale Forschung, Orthopdische Klinik Knig-Ludwig Haus, Wrzburg, Germany; 4Abteilung fr Funktionswerkstoffe der Medizin und der Zahnheilkunde, Universittsklinikum Wrzburg, Wrzburg, Germany


With regard to the growing interest in MR-cell-tracking based on ION-labeled cells a determination of detection limits of IONs by means of MRI and histochemistry remains crucial. We investigated the relation between intracellular iron content (pg/cell) and the capability of histochemistry and MRI to detect in vitro ION-labeled cells. We have shown that MRI is able to detect even low amounts of intracellular VSOP that histochemistry fails to detect. Our results suggest that for the histological validation of MR-cell-tracking studies with VSOP at least 2.13 pg/cell should be achieved.