Accurate localization is key for MR spectra quality and metabolites quantification. Metabolites low concentration and multiple frequencies pose more challenges in-vivo MRS than MRI, due to B0 inhomogeneity, insufficient B1, chemical shift displacement, and artifacts from lipids. Volume selection methods based on overlapping slices improves MRS quality by limiting the region of interest to areas where B0 and B1 can be better controlled. Spatial coverage can be improved by more modern approaches where arbitrary volumes can be shaped with parallel transmit, multiple volumes disentangled by parallel imaged, and different contributions to the MRS signal can be modeled in the reconstruction
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