Visualizing lactate in the ischemic hindleg of mice using localized proton 2D correlation spectroscopy (UHf-P-COSY) at ultrahigh field.
Devashish Das1, Andor Veltien1, and Arend Heerschap1
1Radiology, Radboud University Nijmegen, Nijmegen, Netherlands
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Lactate is associated with mitochondrial and neuromuscular diseases.
Therefore, its methylene (CH2) and methyl (CH3) proton assignment is necessary
in the skeletal muscle. In particular for understanding mechanisms associated
with the skeletal muscle ischemia in rodents. Unambiguous detection of
lactate resonances in the ischemic and/or hypoxic rodent muscle remains
challenging, however, necessary for the assessment of treatment in the
dysfunctional muscle of transgenic animals.
In vivo lactate resonances remain hidden under lipid pool. However, by
tuning spinecho delays in the localized 2D proton correlation sequence we are able
to separate lactate CH2 and CH3 protons from the lipid resonances in the F1
dimension of the 2D-spectrum.
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