Russell W Chan1,2, Alex TL Leong1,2, Patrick P Gao1,2, Leon C Ho1,2, Kevin K Tsia2, and Ed X Wu1,2
1Laboratory of Biomedical Imaging and Signal Processing, The University of Hong Kong, Hong Kong, China, People's Republic of, 2Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, China, People's Republic of
Different layers in mammalian cortex have specific
projections and circuit dynamics. However, how optogenetic stimulation frequencies
at different infragranular layers contribute to widespread and large-scale
cortical and subcortical activities remains largely unexplored. In this study,
optogenetic fMRI is used to investigate layer and frequency dependent
activities by stimulating excitatory neurons in different infragranular layers
of visual
cortex. Our results showed that layer and frequency specific optogenetic stimulation
recruits distinct widespread and large-scale cortical and subcortical
activations. Spatiotemporally varying optogenetic stimulation in combination with
fMRI presents unique opportunities in studying the underlying mechanisms of long-range
neural circuits and brain functional networks.
