Abstract #3680

Hyperpolarized 13C-labeled 5-5-dimethyl-1-pyrroline-N-oxide for in vivo detection of reactive oxygen species

Keita Saito¹, Shingo Matsumoto², Deepak Sail³, Shun Kishimoto¹, Hellmut Merkle⁴, Marcelino Bernardo⁵, Rolf Swenson³, James B. Mitchell¹, and Murali C. Krishna¹

¹Radiation Biology Branch, National Cancer Institute, Bethesda, MD, United States, ²Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan, ³Imaging Probe Development Center, National Heart, Lung, and Blood Institute, Rockville, MD, United States, ⁴National Institute of Neurological Disorder and Stroke, Bethesda, MD, United States, ⁵Molecular Imaging Program, National Cancer Institute, Bethesda, MD, United States

5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) is a spin trap agent used to detect reactive oxygen species (ROS). We synthesized ¹³C-labeled DMPO, and investigated feasibility of hyperpolarized ¹³C-DMPO to detect ROS generated in living animals. Hyperpolarized ¹³C-DMPO gave us a single peak at 76 ppm on the ¹³C-spectrum, and ¹³C-DMPO was distributed through the mouse body immediately after intravenous injection. The results indicate hyperpolarized ¹³C-DMPO provided sufficient magnitude of the ¹³C signal to be detected in the mouse body, and can be applied to some disease models to evaluate the capability for detection of ROS in vivo.

This abstract and the presentation materials are available to members only; a login is required.

Join Here