Anja Bille Bohn1, Nathalie Nielsen2, Christoffer Laustsen2, Hans Stødkilde-Jørgensen2, and Lotte Bonde Bertelsen2
1The department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark, 2MR Research Centre, Aarhus University, Aarhus University Hospital, Aarhus, Denmark
Synopsis:
Studies
of metabolism in stem cells have revealed a shift in the balance between
glycolysis, mitochondrial oxidative phosphorylation and oxidative stress during
the maturation of stem cells. In the stem cells,
pyruvate from glycolysis will mainly be metabolized to lactate as a result of
an uncoupling of the citric acid cycle and the oxidative phosphorylation
pathway, thus the application of a
novel metabolic cell culture tool could add valuable information to the studies
of stem cell characterisation during development. In the present study
we use hyperpolarised [1-13C] pyruvate to characterise mesenchymal stem cells harvested from adipose
tissue.
