Abstract #2010

A quantitative measurement of the cell membrane water permeability of expression-controlled AQP4 cells with diffusion weighted MRI

Takayuki Obata¹, Jeff Kershaw², Yasuhiko Tachibana¹, Youichiro Abe³, Sayaka Shibata², Yoko Ikoma², Hiroshi Kawaguchi⁴, Ichio Aoki², and Masato Yasui³

¹Applied MRI Research, National Institute of Radiological Sciences, Chiba, Japan, ²Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan, ³Department of Pharmacology, Keio University, Tokyo, Japan, ⁴Human Informatics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

We performed multi-b and multi-diffusion-time DWI on aquaporin-4-expressing and non-expressing cells, and demonstrated a clear difference between the signals from the two cell types. The data was interpreted with a two-compartment model including inter-compartmental exchange. It was also assumed that restricted diffusion of water molecules inside the cells leads to the intracellular diffusion coefficient being inversely proportional to the diffusion-time. Estimates of the water-exchange times with this model were comparable with those measured using an independent optical imaging technique, which suggests that this method might be used to characterize cell-membrane water permeability. As the technique can be applied in routine clinical examination, it has the potential to improve clinical diagnosis.

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