Direct Quantitative 13C-Filtered 1H Magnetic Resonance Imaging of Pegylated Biomacromolecules In Vivo
Rohan Alvares1, Justin Lau2,3, Peter Macdonald1, Charles Cunningham2,3, and R. Scott Prosser1
1Department of Chemistry, University of Toronto, Toronto, ON, Canada, 2Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada, 3Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada
We
demonstrate a new platform technology in which macromolecular constituents,
such as proteins and drug delivery systems, are observed directly and
quantitatively in vivo using 1H
MRI of 13C-labeled polyethylene glycol (13C-PEG) tags.
The 28 kDa 13C-PEG tags are non-immunogenic, and each bears
approximately 2500 spectroscopically equivalent 1H nuclei appearing
at a single resonance position. By filtering the 1H PEG signal
through the directly coupled 13C nuclei, background water and fat
signals are largely eliminated. We demonstrate the approach by monitoring in
real-time the distribution of 13C-PEG and 13C-pegylated
albumin injected into the hind leg of a mouse.
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