Meeting Banner
Abstract #3811

Pyruvate Decarboxylase as a Reporter Gene for Magnetic Resonance Spectroscopic Imaging (MRSI)

Piotr Dzien 1 , Sui-Seng Tee 1 , Mikko Kettunen 1 , Timothy Larkin 1 , Scott Lyons 1 , Kerstin Timm 1 , De-En Hu 1 , Tiago Rodrigues 1 , Eva Serrao 1 , Elizabeth Mannion 1 , Paula D'Santos 1 , and Kevin Brindle 1

1 Biochemistry, University of Cambridge, Cambridge, Cambridgeshire, United Kingdom

A reporter gene that could be used with MRI would allow exploitation of the advantages of this imaging technique - spatial and temporal resolution and the penetration depth necessary for in vivo imaging. DNP increases the signal-to-noise ratio sufficiently to allow direct detection of the 13C tracer in real time and presents a novel approach for developing MR reporter genes. Here we report a proof of concept study in which we use inducible expression of a bacterial gene encoding the enzyme, pyruvate decarboxylase (PDC), as a marker for DNP MRSI.

This abstract and the presentation materials are available to members only; a login is required.

Join Here