Abstract #2958
Glial-specific inhibition of TCA cycle in rat, by 1H MRS and 13C MRS
Elise Marie Vinckenbosch 1 , Joo Miguel das Neves Duarte 1,2 , and Rolf Gruetter 1,3
1
Laboratory of functional and metabolic
imaging, Ecole Polytechnique Fdrale de Lausanne,
Lausanne, Switzerland,
2
Faculty
of Biology and Medicine, University of Lausanne,
Switzerland,
3
Department
of Radiology, University of Lausanne and Geneva,
Switzerland
Astrocyte importance in rat brain metabolism was
highlighted by inhibiting glial TCA cycle at the level
of aconitase with fluoroacetate. Changes in
neurochemical profile upon glial-specific inhibition
under hyperglycemia were measured by 1HMRS and brain
compartmentalized metabolism adaptation was studied by
following infused [1,6-13C] glucose degradation with 13C
MRS at 14.1T. Glial aconitase inhibition had effects on
alanine, lactate, aspartate, glutamate and glutamine
brain concentrations. Disruption of ammonia homeostasis
may be rescued by glutamine accumulation upon increased
substrate supply. In 13C MRS, glial selectivity of
fluoroacetate inhibition has been proved and observed
glial metabolic failure didnt affect glutamate TCA
cycle.
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