Abstract #2936
13 C MRS measures the regional changes in neuronal (Glu/GABA) and astroglial mitochondrial TCA cycle and neurotransmitter cycling of the R6/2 Huntingtons disease mice
Golam M. I. Chowdhury 1 , Larry Park 2 , Oxana Lavrova 2 , Monique Thomas 1 , Xiaoxian Ma 3 , Gerard Sanacora 1 , Douglas L Rothman 3 , and Kevin L Behar 1
1
Department of Psychiatry, Yale University
School of Medicine, New Haven, Connecticut, United
States,
2
CHDI
Foundation, Inc, Los Angeles, California, United States,
3
Diagnostic
Radiology, Yale University School of Medicine, New
Haven, Connecticut, United States
Alterations in brain energy metabolism, including
reduced glucose utilization and mitochondrial
respiration, is observed in Huntingtons Disease (HD)
and HD animal models.
1
H-[
13
C]-MRS
can be readily adapted to measure metabolic pathway flux
by use of
13
C
-labeled substrates. In this study we measured the flow
of
13
C
label into glutamate, glutamine and GABA in 6 or 8 week
old R6/2 and control mice following timed intravenous
infusions of [1,6-
13
C
2
]glucose or
[2-
13
C]acetate. Decreased
13
C
labeling was observed at 6 weeks and was more pronounced
in 8 week old R6/2 mice indicating impaired
neuroenergetics and glutamate and GABA neurotransmitter
cycling.
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