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Abstract #2898

STEAM-MiTiS: a new spectral editing method for the detection of scalar coupled metabolites and its application for the detection of Glutamate at 7T

Alessandra Toncelli 1,2 , Ralph Noeske 3 , Mauro Costagli 4 , Valentina Domenici 5 , Gianni Tiberi 4 , Mirco Cosottini 6 , and Michela Tosetti 7

1 Department of Physics, University of Pisa, Pisa, Italy, 2 Sezione di Pisa, INFN, Pisa, Italy, 3 Applied Science Laboratory, GE Healthcare, Berlin, Germany, 4 Fondazione IMAGO7, Italy, 5 Dipartimento di Chimica e Chimica Industriale, University of Pisa, Italy, 6 Dipartimento di Ricerca Traslazionale e delle Nuove Tecnologie in Medicina e Chirurgia, University of Pisa, Italy, 7 Stella Maris Scientific Institute, Italy

We propose a novel spectroscopic method called STEAM-MIxing TIme Subtraction (STEAM-MiTiS) which consists in acquiring two different STEAM spectra with same TE, but different TMs peculiar for each J-coupled metabolite. This technique permits to cancel the contribution of singlet lines and leaves the J-coupled signals, only. The STEAM-MiTiS method has been optimized for the detection of Glutamate. Simulation results yielded the optimum parameter values that we used for both in-vitro and in-vivo validation. Experiments indicated an almost perfect cancellation of singlet and macromolecule signals, and resulted in clear editing of the Glutamate peak whose intensity scaled linearly with its concentration.

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