Meeting Banner
Abstract #2804

MRI Tracking of Transplanted Cell Viability and Function Using a Multimodal Quadruple Fusion Gene Reporter

Anna V Naumova 1,2 , Nathan J Palpant 2,3 , Niranjan Balu 1,2 , Xia Shen 1,4 , Chun Yuan 1,5 , and Charles E Murry 2,3

1 Radiology, University of Washington, Seattle, WA, United States, 2 Center for Cardiovascular Biology, University of Washington, Seattle, WA, United States, 3 Pathology, University of Washington, Seattle, WA, United States, 4 Medicine, University of Washington, Seattle, WA, United States, 5 Bioengineering, University of Washington, Seattle, WA, United States

We propose an integrative approach to monitor transplanted cells non-invasively by combining MRI based structural and functional evaluation with other imaging modalities such as bioluminescence imaging, fluorescence and PET. For this, we engineered a novel genetically-based sensing system consisting of ferritin, luciferase, GCaMP3, and HSV1-tk, which makes graft detectable by multiple imaging modalities. Human embryonic stem cell line expressing quadruple gene reporter demonstrated T2 shortening effect in MRI, strong bioluminescence and fluorescence properties as well as ganciclovir sensitivity. This integrative approach enables longitudinal non-invasive monitoring of the transplanted cells.

This abstract and the presentation materials are available to members only; a login is required.

Join Here