Abstract #2762
Investigation of Cell Membrane Transport and Compartmentalisation of Hyperpolarised Metabolites using a GdDO3A Relaxation Agent
Markus Durst 1,2 , Ulrich Koellisch 1,2 , Francesca Reineri 3 , Valeria Daniele 3 , Concetta Gringeri 4 , Annette Frank 4 , Marion I. Menzel 2 , Axel Haase 1 , Rolf F. Schulte 2 , and Silvio Aime 3
1
IMETUM, Technische Universitt Mnchen,
Garching, Germany,
2
GE
Global Research, Garching, Germany,
3
Department
Chemistry I.F.M. and Molecular Imaging Center,
University of Torino, Torino, Italy,
4
Klinikum
rechts der Isar, Technische Universitt Mnchen, Mnchen,
Germany
Dynamic nuclear polarisation has enabled the analysis of
metabolic pathways in real-time using hyperpolarised
substrates. The conversion of [1-13C]pyruvate to
downstream metabolites such as lactate and alanine can
provide an important tool for studying diseases with
abnormal cellular metabolism such as cancer or ischemia.
In this work, an extracellular relaxation agent (GdDO3A)
was used to quasi-instantaneously quench the
extracellular compartment of the metabolites. In
combination with a substrate saturation sequence,
transporter rates for the cell membrane transport of
hyperpolarised metabolites could be quantified for the
first time in vivo.
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