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Abstract #1517

MR Microscopy and DTI of Organotypic Hippocampal Slice Cultures

Katharina Gbel 1 , Jochen Leupold 1 , Bibek Dhital 1,2 , Pierre LeVan 1 , Marco Reisert 1 , Johannes Gerlach 3 , Robert Kamberger 4 , Carola Haas 3 , Jrgen Hennig 1 , Dominik von Elverfeldt 1 , and Jan G. Korvink 4

1 Medical Physics, Dept. of Radiology, University Medical Center Freiburg, Freiburg, Germany, 2 German Cancer Consortium (DKTK), Heidelberg, Germany, 3 Dept. of Neurosurgery, Experimental Epilepsy Research, University Medical Center Freiburg, Freiburg, Germany, 4 Dept. of Microsystems Engineering (IMTEK), Technical Faculty, University of Freiburg, Freiburg, Germany

Organotypic hippocampal slice cultures are a well established neuronal culture system that combines the advantages of cell culturing with a neuronal network tightly reflecting the in vivo state. They are frequently used to study morphological, molecular and electrophysiological changes associated with epilepsy. Our aim is to investigate these changes during epileptogenesis, particularly using high spatial resolution MR microscopy and DTI which allows continuous monitoring near the cellular level. High resolution MR images of fixed mouse hippocampi were obtained ex vivo and directly compared to histology. First trials in 2D DTI give notion of the structural composition of the hippocampus.

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