Abstract #1517
MR Microscopy and DTI of Organotypic Hippocampal Slice Cultures
Katharina Gbel 1 , Jochen Leupold 1 , Bibek Dhital 1,2 , Pierre LeVan 1 , Marco Reisert 1 , Johannes Gerlach 3 , Robert Kamberger 4 , Carola Haas 3 , Jrgen Hennig 1 , Dominik von Elverfeldt 1 , and Jan G. Korvink 4
1
Medical Physics, Dept. of Radiology,
University Medical Center Freiburg, Freiburg, Germany,
2
German
Cancer Consortium (DKTK), Heidelberg, Germany,
3
Dept.
of Neurosurgery, Experimental Epilepsy Research,
University Medical Center Freiburg, Freiburg, Germany,
4
Dept.
of Microsystems Engineering (IMTEK), Technical Faculty,
University of Freiburg, Freiburg, Germany
Organotypic hippocampal slice cultures are a well
established neuronal culture system that combines the
advantages of cell culturing with a neuronal network
tightly reflecting the in vivo state. They are
frequently used to study morphological, molecular and
electrophysiological changes associated with epilepsy.
Our aim is to investigate these changes during
epileptogenesis, particularly using high spatial
resolution MR microscopy and DTI which allows continuous
monitoring near the cellular level. High resolution MR
images of fixed mouse hippocampi were obtained ex vivo
and directly compared to histology. First trials in 2D
DTI give notion of the structural composition of the
hippocampus.
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