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Abstract #1079

Metabolic profiling of Urinary Bladder Carcinoma Tissues by HRMAS NMR spectroscopy

Abhinav Arun Sonkar 1 , Shatakshi Srivastava 2 , Raja A Roy 2 , Yadvendr A Dheer 1 , and Nuzhat A Husain 3

1 Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India, 2 Center for Biomedical research, SGPGI, Lucknow, Uttar Pradesh, India, 3 Pathology, RMLIMS, Lucknow, Uttar Pradesh, India

In the present work, proton HR-MAS NMR spectroscopic studies of muscle non-invasive Urinary Bladder Carcinoma (UBC) tissue specimens with simultaneous urine analysis has been performed. The detailed metabolic profiling demonstrated significant presence of taurine and branched chain amino acids (BCAA) in tissues of 24 patients suffering from superficial UBC when compared with 29 tissue specimens from the same subjects with non-malignant biopsies. The proton NMR spectra were then subjected to PCA and PLS-DA multivariate analysis. The validated model allowed >85% correct classification of malignant tissues when compared with gold standard histopathological examinations.

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