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Abstract #0773

Differentiating Injured and Normal Lungs by the Ratiometric Analysis of Hyperpolarized-13C-NMR Data

He N. Xu 1,2 , Hoora Shaghaghi 1 , Stephen Kadlececk 1 , Harrilla Profka 1 , Lin Z. Li 1,2 , and Rahim Rizi 1

1 Radiology Department, University of Pennsylvania, Philadelphia, PA, United States, 2 Britton Chance Lab of Redox Imaging, Johnson Research Foundation, University of Pennsylvania, Philadelphia, PA, United States

A non-invasive method for assessing lung injury is needed. We aimed to identify an early metabolic predictor of lung inflammation using the hyperpolarized 13C-NMR technique. Rat lung injury was induced by bleomycin and studied by injecting hyperpolarized 13C-pyruvate to the perfused lung 7 and 21 days after the treatment. The larger rate constants of the LDH-catalyzed reaction quantified by the ratiometric analysis of the time course of lactate/pyruvate ratio indicate inflammation sped up both the forward and reverse reaction rates and altered the cellular redox state. Our results indicate that HP-13C-NMR data can be useful for probing lung disorders.

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