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Abstract #0539

Metabolic flux analysis of acetylcarnitine turnover and mitochondrial oxidation of [2-13C]acetate in rat skeletal muscle in vivo measured by 13C MRS

Jessica A.M. Bastiaansen 1 , Joao M.N. Duarte 1 , and Rolf Gruetter 1,2

1 Laboratory of Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland, 2 Department of Radiology, University of Geneva and University of Lausanne, Switzerland

Acetylcarnitine is a necessary intermediate in acetate metabolism. Time courses of 13C labeled acetylcarnitine would allow for more detailed mathematical models to quantify mitochondrial oxidation of acetate. Using localized DEPT we monitored 13C enrichment and isotope turnover in glutamate and acetylcarnitine in skeletal muscle in vivo following [2-13C]acetate infusion. Two different modeling approaches were evaluated to determine metabolic fluxes, either with or without the 13C labeling of acetylcarnitine and the enzymatic fluxes of acetylCoA synthesase (ACS) and acetylcarnitine transferase (CAT).

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